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Journal of Biomedical Optics

Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy
Author(s): Aaron D. Slepkov; Andrew Ridsdale; Adrian F. Pegoraro; Douglas J. Moffatt; John P. Pezacki; Albert Stolow; Huei-Ning Wan; Ming-Hao Wang; Fu-Jen Kao
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Paper Abstract

We demonstrate the simultaneous collection and separation of femtosecond-laser-based forward-collected coherent anti-Stokes Raman scattering (F-CARS) and two-photon-excitation-induced fluorescence lifetime images (FLIM) using time-correlated single photon counting (TCSPC). We achieve this in a nondescanned geometry using a single multimode fiber without significant loss of light, field of view, and most importantly, TCSPC timing fidelity. In addition to showing the ability to separate CARS images from FLIM images using time gating, we also demonstrate composite multimodal epicollected FLIM imaging with fiber-collected F-CARS imaging in live cells.

Paper Details

Date Published: 1 February 2011
PDF: 7 pages
J. Biomed. Opt. 16(2) 021103 doi: 10.1117/1.3490641
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Aaron D. Slepkov, National Research Council Canada (Canada)
Andrew Ridsdale, National Research Council Canada (Canada)
Adrian F. Pegoraro, National Research Council Canada (Canada)
Douglas J. Moffatt, National Research Council Canada (Canada)
John P. Pezacki, National Research Council Canada (Canada)
Albert Stolow, National Research Council Canada (Canada)
Huei-Ning Wan, National Yang-Ming Univ. (Taiwan)
Ming-Hao Wang, National Yang-Ming Univ. (Taiwan)
Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)

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