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Journal of Biomedical Optics

Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery
Author(s): Yinghua H. Sun; Nisa Hatami; Matthew Yee; Jennifer E. Phipps; Daniel S. Elson; Fredric Gorin; Rudolph J. Schrot; Laura Marcu
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Paper Abstract

We demonstrate for the first time the application of an endoscopic fluorescence lifetime imaging microscopy (FLIM) system to the intraoperative diagnosis of glioblastoma multiforme (GBM). The clinically compatible FLIM prototype integrates a gated (down to 0.2 ns) intensifier imaging system with a fiber-bundle (fiber image guide of 0.5 mm diameter, 10,000 fibers with a gradient index lens objective 0.5 NA, and 4 mm field of view) to provide intraoperative access to the surgical field. Experiments conducted in three patients undergoing craniotomy for tumor resection demonstrate that FLIM-derived parameters allow for delineation of tumor from normal cortex. For example, at 460±25-nm wavelength band emission corresponding to NADH/NADPH fluorescence, GBM exhibited a weaker florescence intensity (35% less, p-value <0.05) and a longer lifetime GBM-Amean=1.59±0.24 ns than normal cortex NC-Amean=1.28±0.04 ns (p-value <0.005). Current results demonstrate the potential use of FLIM as a tool for image-guided surgery of brain tumors.

Paper Details

Date Published: 1 September 2010
PDF: 5 pages
J. Biomed. Opt. 15(5) 056022 doi: 10.1117/1.3486612
Published in: Journal of Biomedical Optics Volume 15, Issue 5
Show Author Affiliations
Yinghua H. Sun, Univ. of California, Davis (United States)
Nisa Hatami, Univ. of California, Davis (United States)
Matthew Yee, Univ. of California, Davis (United States)
Jennifer E. Phipps, Univ. of California, Davis (United States)
Daniel S. Elson, Imperial College London (United Kingdom)
Fredric Gorin, Univ. of California, Davis (United States)
Rudolph J. Schrot, Univ. of California, Davis (United States)
Laura Marcu, Univ. of California, Davis (United States)

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