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Journal of Biomedical Optics

Human graft cornea and laser incisions imaging with micrometer scale resolution full-field optical coherence tomography
Author(s): Gaël Latour; Gaelle Georges; Laure Siozade Lamoine; Carole Deumié-Raviol; John Conrath; Louis Hoffart
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Paper Abstract

Micrometer scale resolution full-field optical coherence tomography (FF-OCT) is developed for imaging human graft corneas. Three-dimensional (3-D) images with ultrahigh resolution (respectively, 1 and 1.5 µm in the axial and transverse directions), comparable to traditional histological sections, are obtained allowing the visualization of the cells and the precise structure of the different layers that compose the tissue. The sensitivity of our device enables imaging the entire thickness of the cornea, even in edematous corneas more than 800 µm thick. Furthermore, we provide tomographic 3-D images of laser incisions inside the tissue at various depths without slicing the studied corneas. The effects of laser ablations can be observed, along various optical sections, directly in the bulk of the sample with high accuracy, providing information on the interface quality and also imaging tiny changes of the tissue structure. FF-OCT appears to be a powerful tool for subcellular imaging of the corneal structure and pathologies on the entire thickness of the tissue as well as interface quality and changes in the collagen structure due to laser incisions on ex vivo human cornea.

Paper Details

Date Published: 1 September 2010
PDF: 9 pages
J. Biomed. Opt. 15(5) 056006 doi: 10.1117/1.3486544
Published in: Journal of Biomedical Optics Volume 15, Issue 5
Show Author Affiliations
Gaël Latour, Institut Fresnel (France)
Gaelle Georges, Institut Fresnel (France)
Laure Siozade Lamoine, Institut Fresnel (France)
Carole Deumié-Raviol, Institut Fresnel (France)
John Conrath, Hôpital de la Timone (France)
Louis Hoffart, Hôpital de la Timone (France)

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