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Journal of Biomedical Optics

Comparative study of photothermolysis of cancer cells with nuclear-targeted or cytoplasm-targeted gold nanospheres: continuous wave or pulsed lasers
Author(s): Xiaohua Huang; Bin Kang; Wei Qian; Megan A. Mackey; Po C. Chen; Adegboyega K. Oyelere; Ivan H. El-Sayed; Mostafa A. El-Sayed
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Paper Abstract

We conduct a comparative study on the efficiency and cell death pathways of continuous wave (cw) and nanosecond pulsed laser photothermal cancer therapy using gold nanospheres delivered to either the cytoplasm or nucleus of cancer cells. Cytoplasm localization is achieved using arginine-glycine-aspartate peptide modified gold nanospheres, which target integrin receptors on the cell surface and are subsequently internalized by the cells. Nuclear delivery is achieved by conjugating the gold nanospheres with nuclear localization sequence peptides originating from the simian virus. Photothermal experiments show that cell death can be induced with a single pulse of a nanosecond laser more efficiently than with a cw laser. When the cw laser is applied, gold nanospheres localized in the cytoplasm are more effective in inducing cell destruction than gold nanospheres localized at the nucleus. The opposite effect is observed when the nanosecond pulsed laser is used, suggesting that plasmonic field enhancement of the nonlinear absorption processes occurs at high localization of gold nanospheres at the nucleus. Cell death pathways are further investigated via a standard apoptosis kit to show that the cell death mechanisms depend on the type of laser used. While the cw laser induces cell death via apoptosis, the nanosecond pulsed laser leads to cell necrosis. These studies add mechanistic insight to gold nanoparticle-based photothermal therapy of cancer.

Paper Details

Date Published: 1 September 2010
PDF: 7 pages
J. Biomed. Opt. 15(5) 058002 doi: 10.1117/1.3486538
Published in: Journal of Biomedical Optics Volume 15, Issue 5
Show Author Affiliations
Xiaohua Huang, Georgia Institute of Technology (United States)
Bin Kang, Georgia Institute of Technology (United States)
Wei Qian, Georgia Institute of Technology (United States)
Megan A. Mackey, Georgia Institute of Technology (United States)
Po C. Chen, Georgia Institute of Technology (United States)
Adegboyega K. Oyelere, Georgia Institute of Technology (United States)
Ivan H. El-Sayed, Univ. of California, San Francisco (United States)
Mostafa A. El-Sayed, Georgia Institute of Technology (United States)

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