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Journal of Biomedical Optics

Dynamic quantitative photothermal monitoring of cell death of individual human red blood cells upon glucose depletion
Author(s): Srivathsan Vasudevan; George Chung Kit Chen; Marta Andika; Shuchi Agarwal; Peng Chen; Malini C. Olivo
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Paper Abstract

Red blood cells (RBCs) have been found to undergo "programmed cell death," or eryptosis, and understanding this process can provide more information about apoptosis of nucleated cells. Photothermal (PT) response, a label-free photothermal noninvasive technique, is proposed as a tool to monitor the cell death process of living human RBCs upon glucose depletion. Since the physiological status of the dying cells is highly sensitive to photothermal parameters (e.g., thermal diffusivity, absorption, etc.), we applied linear PT response to continuously monitor the death mechanism of RBC when depleted of glucose. The kinetics of the assay where the cell's PT response transforms from linear to nonlinear regime is reported. In addition, quantitative monitoring was performed by extracting the relevant photothermal parameters from the PT response. Twofold increases in thermal diffusivity and size reduction were found in the linear PT response during cell death. Our results reveal that photothermal parameters change earlier than phosphatidylserine externalization (used for fluorescent studies), allowing us to detect the initial stage of eryptosis in a quantitative manner. Hence, the proposed tool, in addition to detection of eryptosis earlier than fluorescence, could also reveal physiological status of the cells through quantitative photothermal parameter extraction.

Paper Details

Date Published: 1 September 2010
PDF: 9 pages
J. Biomed. Opt. 15(5) 057001 doi: 10.1117/1.3484260
Published in: Journal of Biomedical Optics Volume 15, Issue 5
Show Author Affiliations
Srivathsan Vasudevan, Nanyang Technological Univ. (Singapore)
George Chung Kit Chen, Nanyang Technological Univ. (Singapore)
Marta Andika, Nanyang Technological Univ. (Singapore)
Shuchi Agarwal, Nanyang Technological Univ. (Singapore)
Peng Chen, Nanyang Technological Univ. (Singapore)
Malini C. Olivo, Singapore Bioimaging Consortium (Singapore)

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