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Journal of Biomedical Optics • Open Access

On the use of fluorescence probes for detecting reactive oxygen and nitrogen species associated with photodynamic therapy
Author(s): Michael Price; David H. Kessel

Paper Abstract

Fluorescent probes are frequently employed for the detection of different reactive oxygen and nitrogen species formed during the irradiation of photosensitized cells and tissues. Investigators often interpret the results in terms of information provided with the different probes without examining specificity or determinants of fluorogenic reactions. We examine five fluorescent probes in a cell-free system: reduced 2,7-dichlorofluorescein, dihydroethidine, dihydrorhodamine, 3-(p aminophenyl) fluorescein (APF), and 4,5-diaminofluorescein. Of these, only APF demonstrates a high degree of specificity for a single reactive species. There is a substantial influence of peroxidase activity on all fluorogenic interactions. The fluorescence of the photosensitizing agent also must be taken into account in evaluating results.

Paper Details

Date Published: 1 September 2010
PDF: 3 pages
J. Biomed. Opt. 15(5) 051605 doi: 10.1117/1.3484258
Published in: Journal of Biomedical Optics Volume 15, Issue 5
Show Author Affiliations
Michael Price, Wayne State Univ. (United States)
David H. Kessel, Wayne State Univ. School of Medicine (United States)


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