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Journal of Biomedical Optics • Open Access

Acquiring 3-D information about thick objects from differential interference contrast images using texture extraction

Paper Abstract

The extraction of 3-D morphological information about thick objects is explored in this work. We extract this information from 3-D differential interference contrast (DIC) images by applying a texture detection method. Texture extraction methods have been successfully used in different applications to study biological samples. A 3-D texture image is obtained by applying a local entropy-based texture extraction method. The use of this method to detect regions of blastocyst mouse embryos that are used in assisted reproduction techniques such as in vitro fertilization is presented as an example. Results demonstrate the potential of using texture detection methods to improve morphological analysis of thick samples, which is relevant to many biomedical and biological studies. Fluorescence and optical quadrature microscope phase images are used for validation.

Paper Details

Date Published: 1 July 2010
PDF: 7 pages
J. Biomed. Opt. 15(4) 046021 doi: 10.1117/1.3475961
Published in: Journal of Biomedical Optics Volume 15, Issue 4
Show Author Affiliations
Heidy Sierra, Northeastern Univ. (United States)
Dana H. Brooks, Northeastern Univ. (United States)
Charles A. DiMarzio, Northeastern Univ. (United States)

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