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Journal of Biomedical Optics

Shack-Hartmann wavefront-sensor-based adaptive optics system for multiphoton microscopy
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Paper Abstract

The imaging depth of two-photon excitation fluorescence microscopy is partly limited by the inhomogeneity of the refractive index in biological specimens. This inhomogeneity results in a distortion of the wavefront of the excitation light. This wavefront distortion results in image resolution degradation and lower signal level. Using an adaptive optics system consisting of a Shack-Hartmann wavefront sensor and a deformable mirror, wavefront distortion can be measured and corrected. With adaptive optics compensation, we demonstrate that the resolution and signal level can be better preserved at greater imaging depth in a variety of ex-vivo tissue specimens including mouse tongue muscle, heart muscle, and brain. However, for these highly scattering tissues, we find signal degradation due to scattering to be a more dominant factor than aberration.

Paper Details

Date Published: 1 July 2010
PDF: 10 pages
J. Biomed. Opt. 15(4) 046022 doi: 10.1117/1.3475954
Published in: Journal of Biomedical Optics Volume 15, Issue 4
Show Author Affiliations
Jae-Won Cha, Massachusetts Institute of Technology (United States)
Jérôme Ballesta, Imagine Optic Inc. (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)


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