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Journal of Biomedical Optics • Open Access

Cell deformation cytometry using diode-bar optical stretchers
Author(s): Ihab Sraj; Charles D. Eggleton; Ralph Jimenez; Erich E. Hoover; Jeffrey A. Squier; Justin Chichester; David W. Marr

Paper Abstract

The measurement of cell elastic parameters using optical forces has great potential as a reagent-free method for cell classification, identification of phenotype, and detection of disease; however, the low throughput associated with the sequential isolation and probing of individual cells has significantly limited its utility and application. We demonstrate a single-beam, high-throughput method where optical forces are applied anisotropically to stretch swollen erythrocytes in microfluidic flow. We also present numerical simulations of model spherical elastic cells subjected to optical forces and show that dual, opposing optical traps are not required and that even a single linear trap can induce cell stretching, greatly simplifying experimental implementation. Last, we demonstrate how the elastic modulus of the cell can be determined from experimental measurements of the equilibrium deformation. This new optical approach has the potential to be readily integrated with other cytometric technologies and, with the capability of measuring cell populations, enabling true mechanical-property-based cell cytometry.

Paper Details

Date Published: 1 July 2010
PDF: 7 pages
J. Biomed. Opt. 15(4) 047010 doi: 10.1117/1.3470124
Published in: Journal of Biomedical Optics Volume 15, Issue 4
Show Author Affiliations
Ihab Sraj, Univ. of Maryland, Baltimore County (United States)
Charles D. Eggleton, Univ. of Maryland, Baltimore County (United States)
Ralph Jimenez, JILA (United States)
Erich E. Hoover, Colorado School of Mines (United States)
Jeffrey A. Squier, Colorado School of Mines (United States)
Justin Chichester, Colorado School of Mines (United States)
David W. Marr, Colorado School of Mines (United States)


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