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Journal of Biomedical Optics

Silver nanoparticle-induced degranulation observed with quantitative phase microscopy
Author(s): Wenzhong Yang; Seungrag Lee; Jiyong Lee; Yoon Sung Bae; Dug-Young Kim
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Paper Abstract

Monitoring a degranulation process in a live mast cell is a quite important issue in immunology and pharmacology. Because the size of a granule is normally much smaller than the resolution limit of an optical microscope system, there is no direct real-time live cell imaging technique for observing degranulation processes except for fluorescence imaging techniques. In this research, we propose optical quantitative phase microscopy (QPM) as a new observation tool to study degranulation processes in a live mast cell without any fluorescence labeling. We measure the cell volumes and the cross sectional profiles (x-z plane) of an RBL-2H3 cell and a HeLa cell, before and after they are exposed to calcium ionophore A23187 and silver nanoparticles (AgNPs). We verify that the volume and the cross sectional line profile of the RBL-2H3 cell were changed significantly when it was exposed to A23187. When 50 µg/mL of AgNP is used instead of A23187, the measurements of cell volume and cross sectional profiles indicate that RBL-2H3 cells also follow degranulation processes. Degranulation processes for these cells are verified by monitoring the increase of intracellular calcium ([Ca2+]i) and histamine with fluorescent methods.

Paper Details

Date Published: 1 July 2010
PDF: 10 pages
J. Biomed. Opt. 15(4) 045005 doi: 10.1117/1.3470104
Published in: Journal of Biomedical Optics Volume 15, Issue 4
Show Author Affiliations
Wenzhong Yang, Gwangju Institute of Science and Technology (Korea, Republic of)
Seungrag Lee, Gwangju Institute of Science and Technology (Korea, Republic of)
Jiyong Lee, Gwangju Institute of Science and Technology (Korea, Republic of)
Yoon Sung Bae, Gwangju Institute of Science and Technology (Korea, Republic of)
Dug-Young Kim, Gwangju Institute of Science and Technology (Korea, Republic of)


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