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Journal of Biomedical Optics • Open Access

Direct detection of aptamer-thrombin binding via surface-enhanced Raman spectroscopy
Author(s): Cynthia V. Pagba; Stephen M. Lane; Hansang Cho; Sebastian Wachsmann-Hogiu

Paper Abstract

In this study, we exploit the sensitivity offered by surface-enhanced Raman scattering (SERS) for the direct detection of thrombin using the thrombin-binding aptamer (TBA) as molecular receptor. The technique utilizes immobilized silver nanoparticles that are functionalized with thiolated thrombin-specific binding aptamer, a 15-mer (5'-GGTTGGTGTGGTTGG-3') quadruplex forming oligonucleotide. In addition to the Raman vibrational bands corresponding to the aptamer and blocking agent, new peaks (mainly at 1140, 1540, and 1635 cm−1) that are characteristic of the protein are observed upon binding of thrombin. These spectral changes are not observed when the aptamer-nanoparticle assembly is exposed to a nonbinding protein such as bovine serum albumin (BSA). This methodology could be further used for the development of label-free biosensors for direct detection of proteins and other molecules of interest for which aptamers are available.

Paper Details

Date Published: 1 July 2010
PDF: 8 pages
J. Biomed. Opt. 15(4) 047006 doi: 10.1117/1.3465594
Published in: Journal of Biomedical Optics Volume 15, Issue 4
Show Author Affiliations
Cynthia V. Pagba, UC Davis Medical Ctr. (United States)
Stephen M. Lane, UC Davis Medical Ctr. (United States)
Hansang Cho, Univ. of California, Berkeley (United States)
Sebastian Wachsmann-Hogiu, NSF Ctr. for Biophotonics Science and Technology (United States)


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