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Journal of Biomedical Optics • Open Access

Scanning elastic scattering spectroscopy detects metastatic breast cancer in sentinel lymph nodes
Author(s): Martin R. Austwick; Benjamin R. Clark; Charles A. Mosse; Kristie S. Johnson; D. Wayne Chicken; Santosh K. Somasundaram; Katherine Calabro; Ying Zhu; Mary Falzon; Gabrijela Kocjan; Thomas Fearn; Stephen G. Bown; Irving J. Bigio; Mohammed R. S. Keshtgar

Paper Abstract

A novel method for rapidly detecting metastatic breast cancer within excised sentinel lymph node(s) of the axilla is presented. Elastic scattering spectroscopy (ESS) is a point-contact technique that collects broadband optical spectra sensitive to absorption and scattering within the tissue. A statistical discrimination algorithm was generated from a training set of nearly 3000 clinical spectra and used to test clinical spectra collected from an independent set of nodes. Freshly excised nodes were bivalved and mounted under a fiber-optic plate. Stepper motors raster-scanned a fiber-optic probe over the plate to interrogate the node's cut surface, creating a 20×20 grid of spectra. These spectra were analyzed to create a map of cancer risk across the node surface. Rules were developed to convert these maps to a prediction for the presence of cancer in the node. Using these analyses, a leave-one-out cross-validation to optimize discrimination parameters on 128 scanned nodes gave a sensitivity of 69% for detection of clinically relevant metastases (71% for macrometastases) and a specificity of 96%, comparable to literature results for touch imprint cytology, a standard technique for intraoperative diagnosis. ESS has the advantage of not requiring a pathologist to review the tissue sample.

Paper Details

Date Published: 1 July 2010
PDF: 8 pages
J. Biomed. Opt. 15(4) 047001 doi: 10.1117/1.3463005
Published in: Journal of Biomedical Optics Volume 15, Issue 4
Show Author Affiliations
Martin R. Austwick, Univ. College London (United Kingdom)
Benjamin R. Clark, Univ. College London (United Kingdom)
Charles A. Mosse, Univ. College London (United Kingdom)
Kristie S. Johnson, Univ. College London (United Kingdom)
D. Wayne Chicken, Univ. College London (United Kingdom)
Santosh K. Somasundaram, Univ. College London (United Kingdom)
Katherine Calabro, Boston Univ. (United States)
Ying Zhu, Univ. College London (United Kingdom)
Mary Falzon, Royal Free and Univ. College Medical School (United Kingdom)
Gabrijela Kocjan, Univ. College London (United Kingdom)
Thomas Fearn, Univ. College London (United Kingdom)
Stephen G. Bown, Univ. College London (United Kingdom)
Irving J. Bigio, Boston Univ. (United States)
Mohammed R. S. Keshtgar, Univ. College London (United Kingdom)

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