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Journal of Biomedical Optics

Quantitative visualization of colloidal and intracellular gold nanoparticles by confocal microscopy
Author(s): Sabine Klein; Svea Petersen; Ulrike Taylor; Detlef Rath; Stephan Barcikowski
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Paper Abstract

Gold nanoparticles (AuNPs) have the potential to become a versatile biomarker. For further use of AuNPs labeled with functionalized molecules, their visualization in biological systems by routine laboratory tools such as light microscopy is crucial. However, the size far below the diffraction limit affords specialized parameters for microscopical detection, which stimulated the current study, aimed to determine from which size onward AuNPs, either in dispersion or cell-associated, can be reliably detected by standard confocal microscopy. First, gold colloids of size-restricted fractions are examined in dispersion. At a minimum particle size of 60 nm, detection appears to be reliable. Particle counts in dilution series confirm these results by revealing single particle detection of 60-nm colloids. Second, AuNPs are visualized and quantified in cells, which interestingly cause a phase shift in the reflection of AuNPs. Gold mass spectroscopy confirms the number of AuNPs counted microscopically inside cells. Furthermore, it demonstrates for the first time a very high diffusion rate of 15-nm particles into the cells. In conclusion, the results back the suitability of confocal microscopy for the quantitative tracking of colloidal and intracellular gold nanoparticles sized 60 nm.

Paper Details

Date Published: 1 May 2010
PDF: 11 pages
J. Biomed. Opt. 15(3) 036015 doi: 10.1117/1.3461170
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Sabine Klein, Friedrich-Loeffler-Institut (Germany)
Svea Petersen, Laser Zentrum Hannover e.V. (Germany)
Ulrike Taylor, Friedrich-Loeffler-Institut (Germany)
Detlef Rath, Friedrich-Loeffler-Institut (Germany)
Stephan Barcikowski, Laser Zentrum Hannover e.V. (Germany)


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