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Journal of Biomedical Optics

Volumetric in vivo imaging of intracochlear microstructures in mice by high-speed spectral domain optical coherence tomography
Author(s): Hrebesh M. Subhash; Viviana Davila; Hai Sun; Anh T. Nguyen-Huyn; Alfred L. Nuttall; Ruikang K. Wang
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Paper Abstract

There is considerable interest in developing new methods for in vivo imaging of the complex anatomy of the mammalian cochlea for clinical as well as fundamental studies. In this study, we explored, the feasibility of spectral domain optical coherence tomography (SD-OCT) for 3-D in vivo imaging of the cochlea in mice. The SD-OCT system employed in this study used a broadband light source centered at 1300 nm, and the imaging speed of the system was 47,000 A-scans per second using the InGaAs camera. The system was capable of providing fully processed, high-resolution B-scan images [512 (axial)×128 (lateral) pixels] at 280 frames per sec. The 3-D imaging acquisition time for a whole cochlea was ~0.45 sec. The traditional SD-OCT structural imaging algorithm was used to reconstruct 3-D cochlear morphology. We demonstrated that SD-OCT can be successfully used for in vivo imaging of important morphological features within the mouse cochlea, such as the otic capsule and structures within, including Reissner's membrane, the basilar membrane, tectorial membrane, organ of Corti, and modiolus of the apical and middle turns.

Paper Details

Date Published: 1 May 2010
PDF: 7 pages
J. Biomed. Opt. 15(3) 036024 doi: 10.1117/1.3456554
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Hrebesh M. Subhash, Oregon Health & Science Univ. (United States)
Viviana Davila, Oregon Health & Science Univ. (United States)
Hai Sun, Oregon Health & Science Univ. (United States)
Anh T. Nguyen-Huyn, Oregon Health & Science Univ. (United States)
Alfred L. Nuttall, Oregon Health & Science Univ. (United States)
Ruikang K. Wang, Oregon Health & Science Univ. (United States)


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