Share Email Print

Journal of Biomedical Optics

Quantitative Raman spectroscopy in turbid media
Author(s): Carina Reble; Ingo H. Gersonde; Stefan Andree; Hans-Joachim Eichler; Jürgen Helfmann
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Intrinsic Raman spectra of biological tissue are distorted by the influences of tissue absorption and scattering, which significantly challenge signal quantification. A combined Raman and spatially resolved reflectance setup is introduced to measure the absorption coefficient µa and the reduced scattering coefficient µ of the tissue, together with the Raman signals. The influence of µa and µ on the resonance Raman signal of -carotene is measured at 1524 cm−1 by tissue phantom measurements and Monte Carlo simulations for µa=0.01 to 10 mm−1 and µ=0.1 to 10 mm−1. Both methods show that the Raman signal drops roughly proportional to 1/µa for µa>0.2 mm−1 in the measurement geometry and that the influence of µ is weaker, but not negligible. Possible correction functions dependent on the elastic diffuse reflectance are investigated to correct the Raman signal for the influence of µa and µ, provided that µa and µ are measured as well. A correction function based on the Monte Carlo simulation of Raman signals is suggested as an alternative. Both approaches strongly reduce the turbidity-induced variation of the Raman signals and allow absolute Raman scattering coefficients to be determined.

Paper Details

Date Published: 1 May 2010
PDF: 8 pages
J. Biomed. Opt. 15(3) 037016 doi: 10.1117/1.3456370
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Carina Reble, Laser- und Medizin-Technologie GmbH, Berlin (Germany)
Ingo H. Gersonde, Laser- und Medizin-Technologie GmbH, Berlin (Germany)
Stefan Andree, Laser- und Medizin-Technologie GmbH, Berlin (Germany)
Hans-Joachim Eichler, Technische Univ. Berlin (Germany)
Jürgen Helfmann, Laser- und Medizin-Technologie GmbH, Berlin (Germany)

© SPIE. Terms of Use
Back to Top