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Journal of Biomedical Optics • Open Access

Speckle fluctuation spectroscopy of intracellular motion in living tissue using coherence-domain digital holography
Author(s): Kwan Jeong; John J. Turek; David D. Nolte

Paper Abstract

Dynamic speckle from 3-D coherence-gated optical sections provides a sensitive label-free measure of cellular activity up to 1 mm deep in living tissue. However, specificity to cellular functionality has not previously been demonstrated. In this work, we perform fluctuation spectroscopy on dynamic light scattering captured using coherence-domain digital holography to obtain the spectral response of tissue that is perturbed by temperature, osmolarity, and antimitotic cytoskeletal drugs. Different perturbations induce specific spectrogram response signatures that can show simultaneous enhancement and suppression in different spectral ranges.

Paper Details

Date Published: 1 May 2010
PDF: 3 pages
J. Biomed. Opt. 15(3) 030514 doi: 10.1117/1.3456369
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Kwan Jeong, Korea Military Academy (Korea, Republic of)
John J. Turek, Purdue Univ. (United States)
David D. Nolte, Purdue Univ. (United States)

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