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Journal of Biomedical Optics

Characteristics of calcium signaling in astrocytes induced by photostimulation with femtosecond laser
Author(s): Yuan Zhao; Yuan Zhang; Wei Zhou; Xiuli Liu; Shaoqun Zeng; Qingming Luo
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Paper Abstract

Astrocytes have been identified to actively contribute to brain functions through Ca2+ signaling, serving as a bridge to communicate with neurons and other brain cells. However, conventional stimulation techniques are hard to apply to delicate investigations on astrocytes. Our group previously reported photostimulation with a femtosecond laser to evoke astrocytic calcium (Ca2+) waves, providing a noninvasive and efficient approach with highly precise targeting. In this work, detailed characteristics of astrocytic Ca2+ signaling induced by photostimulation are presented. In a purified astrocytic culture, after the illumination of a femtosecond laser onto one cell, a Ca2+ wave throughout the network with reduced speed is induced, and intracellular Ca2+ oscillations are observed. The intercellular propagation is pharmacologically confirmed to be mainly mediated by ATP through P2Y receptors. Different patterns of Ca2+ elevations with increased amplitude in the stimulated astrocyte are discovered by varying the femtosecond laser power, which is correspondingly followed by broader intercellular waves. These indicate that the strength of photogenerated Ca2+ signaling in astrocytes has a positive relationship with the stimulating laser power. Therefore, distinct Ca2+ signaling is feasibly available for specific studies on astrocytes by employing precisely controlled photostimulation.

Paper Details

Date Published: 1 May 2010
PDF: 5 pages
J. Biomed. Opt. 15(3) 035001 doi: 10.1117/1.3454390
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Yuan Zhao, Wuhan National Lab. for Optoelectronics (China)
Yuan Zhang, Huazhong Univ. of Science and Technology (China)
Wei Zhou, Wuhan National Lab. for Optoelectronics (China)
Xiuli Liu, Wuhan National Lab. for Optoelectronics (China)
Shaoqun Zeng, Britton Chance Ctr. for Biomedical Photonics (China)
Qingming Luo, Britton Chance Ctr. for Biomedical Photonics (China)


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