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Journal of Biomedical Optics • Open Access

Murine photoplethysmography for in vivo estimation of vascular gold nanoshell concentration
Author(s): Gregory J. Michalak; Glenn P. Goodrich; Jon A. Schwartz; William D. James

Paper Abstract

There is an urgent clinical need to monitor the intravenous delivery and bioavailability of circulating nanoparticles used in cancer therapy. This work presents the use of photoplethysmography for the noninvasive real-time estimation of vascular gold nanoshell concentration in a murine subject. We develop a pulse photometer capable of accurately measuring the photoplethysmogram in mice and determining the ratio of pulsatile changes in optical extinction between 805 and 940 nm, commonly referred to as R. These wavelengths are selected to correspond to the extinction properties of gold nanoshells. Six 30-s measurements (5 min, 2, 4, 6, 8, 10 h) are taken under light anesthesia to observe the change in R as the nanoparticles clear from the circulation. Our model describes the linear fit (R2=0.85) between R and the concentration of nanoparticles measured via ex vivo spectrophotometric and instrumental neutron activation analysis. This demonstrates the utility of this technique in support of clinical nanoparticle therapies.

Paper Details

Date Published: 1 July 2010
PDF: 5 pages
J. Biomed. Opt. 15(4) 047007 doi: 10.1117/1.3454374
Published in: Journal of Biomedical Optics Volume 15, Issue 4
Show Author Affiliations
Gregory J. Michalak, Louisiana Tech Univ. (United States)
Glenn P. Goodrich, Nanospectra Biosciences, Inc. (United States)
Jon A. Schwartz, Nanospectra Biosciences, Inc. (United States)
William D. James, Texas A&M Univ. (United States)

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