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Journal of Biomedical Optics • Open Access

Imaging targeted-agent binding in vivo with two probes
Author(s): Brian W. Pogue; Kimberley S. Samkoe; Shannon K. Hextrum; Julia A. O'Hara; Michael Jermyn; Subhadra Srinivasan; Tayyaba Hasan

Paper Abstract

An approach to quantitatively image targeted-agent binding rate in vivo is demonstrated with dual-probe injection of both targeted and nontargeted fluorescent dyes. Images of a binding rate constant are created that reveal lower than expected uptake of epidermal growth factor in an orthotopic xenograft pancreas tumor (2.3×10−5 s−1), as compared to the normal pancreas (3.4×10−5 s−1). This approach allows noninvasive assessment of tumor receptor targeting in vivo to determine the expected contrast, spatial localization, and efficacy in therapeutic agent delivery.

Paper Details

Date Published: 1 May 2010
PDF: 3 pages
J. Biomed. Opt. 15(3) 030513 doi: 10.1117/1.3449109
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Brian W. Pogue, Dartmouth College (United States)
Kimberley S. Samkoe, Dartmouth College (United States)
Shannon K. Hextrum, Dartmouth College (United States)
Julia A. O'Hara, Dartmouth Medical School (United States)
Michael Jermyn
Subhadra Srinivasan, Dartmouth College (United States)
Tayyaba Hasan, Massachusetts General Hospital (United States)

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