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Journal of Biomedical Optics

Optical waveguides formed by silver ion exchange in Schott SG11 glass for waveguide evanescent field fluorescence microscopy: evanescent images of HEK293 cells
Author(s): Abdollah Hassanzadeh; Michael Nitsche; Souzan Armstrong; Noushin Nabavi; Rene Harrison; S. Jeffrey Dixon; Uwe Langbein; Silvia Mittler
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Paper Abstract

Planar glass waveguides with a specific number of modes were fabricated by Ag+-Na+ exchange in Schott SG11 glass. The effective refractive indices were determined using m-line spectroscopy in both s- and p-polarization. By using the reversed Wentzel-Kramers-Brillouin approximation, the index profiles were described by a nonlinear diffusion equation. The diffusion coefficients for Ag+ were established, as well as the penetration depth of the evanescent field in an aqueous environment for the different modes. The integrals of |E|2 fields for the evanescent-guided fields were investigated. These are important when evanescent fields are used for illumination in interface microscopy, an alternative method to total internal reflection fluorescence (TIRF) microscopy. The photoluminescent behavior of the waveguides was investigated as a function of ion exchange time and excitation wavelengths. Comparable images were obtained of fluorescently labeled HEK293 cells using TIRF microscopy and waveguide evanescent field fluorescence microscopy. Imaging was performed using HEK293 cells, delivering similar images and information.

Paper Details

Date Published: 1 May 2010
PDF: 7 pages
J. Biomed. Opt. 15(3) 036018 doi: 10.1117/1.3443796
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Abdollah Hassanzadeh, The Univ. of Western Ontario (Canada)
Michael Nitsche, The Univ. of Western Ontario (Canada)
Souzan Armstrong, The Univ. of Western Ontario (Canada)
Noushin Nabavi, Univ. of Toronto Scarborough (Canada)
Rene Harrison, Univ. of Toronto Scarborough (Canada)
S. Jeffrey Dixon, The Univ. of Western Ontario (Canada)
Uwe Langbein, Fachhochschule Wiesbaden (Germany)
Silvia Mittler, The Univ. of Western Ontario (Canada)


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