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Journal of Biomedical Optics

Biochemical analysis and quantification of hematopoietic stem cells by infrared spectroscopy
Author(s): Udi Zelig; Ziv Dror; Svetlana Iskovich; Amir Zwielly; Miri Ben-Harush; Ilana Nathan; Shaul Mordechai; Joseph Kapelushnik
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Paper Abstract

Identification of hematopoietic stem cells (HSCs) in different stages of maturation is one of the major issues in stem cell research and bone marrow (BM) transplantation. Each stage of maturation of HSCs is characterized by a series of distinct glycoproteins present on the cell plasma membrane surface, named a cluster of differentiation (CD). Currently, complicated and expensive procedures based on CD expression are needed for identification and isolation of HSCs. This method is under dispute, since the correct markers' composition is not strictly clear, thus there is need for a better method for stem cell characterization. In the present study, Fourier transform infrared (FTIR) spectroscopy is employed as a novel optical method for identification and characterization of HSCs based on their entire biochemical features. FTIR spectral analysis of isolated mice HSCs reveals several spectral markers related to lipids, nucleic acids, and carbohydrates, which distinguish HSCs from BM cells. The unique "open" conformation of HSC DNA as identified by FTIR is exploited for HSCs quantification in the BM. The proposed method of FTIR spectroscopy for HSC identification and quantification can contribute to stem cell research and BM transplantation.

Paper Details

Date Published: 1 May 2010
PDF: 8 pages
J. Biomed. Opt. 15(3) 037008 doi: 10.1117/1.3442728
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Udi Zelig, Ben-Gurion Univ. of the Negev (Israel)
Ziv Dror, Soroka Univ. Medical Ctr. (Israel)
Svetlana Iskovich, Schneider Children's Medical Ctr. of Israel (Israel)
Amir Zwielly, Ben-Gurion Univ. of the Negev (Israel)
Miri Ben-Harush, Soroka Univ. Medical Ctr. (Israel)
Ilana Nathan, Ben-Gurion Univ. of the Negev (Israel)
Shaul Mordechai, Ben-Gurion Univ. of the Negev (Israel)
Joseph Kapelushnik, Soroka Univ. Medical Ctr. (Israel)

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