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Journal of Biomedical Optics

Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy
Author(s): Wen Lo; Tsung-Jen Wang; Wei-Liang Chen; Chiu-Mei Hsueh; Shean-Jen Chen; Yang-Fang Chen; Hsiu-Chu Chou; Pi-Jung Lin; Fung-Rong Hu; Chen-Yuan Dong
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Paper Abstract

We applied multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) microscopy to monitor corneal wound healing after photorefractive keratectomy (PRK). Our results show that keratocyte activation can be observed by an increase in its MAF, while SHG imaging of corneal stroma can show the depletion of Bowman's layer after PRK and the reticular collagen deposition in the wound healing stage. Furthermore, quantification of the keratocyte activation and collagen deposition in conjunction with immunohistochemistry and histological images demonstrate the effectiveness of mitomycin C (MMC) in suppressing myofibroblast proliferation and collagen regeneration in the post-PRK wound healing process.

Paper Details

Date Published: 1 May 2010
PDF: 7 pages
J. Biomed. Opt. 15(3) 036005 doi: 10.1117/1.3432718
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Wen Lo, National Taiwan Univ. (Taiwan)
Tsung-Jen Wang, Taipei Medical Univ. Hospital (Taiwan)
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Chiu-Mei Hsueh, National Taiwan Univ. (Taiwan)
Shean-Jen Chen, National Cheng Kung Univ. (Taiwan)
Yang-Fang Chen, National Taiwan Univ. (Taiwan)
Hsiu-Chu Chou, Taipei Medical Univ. (Taiwan)
Pi-Jung Lin, National Taiwan Univ. Hospital (Taiwan)
Fung-Rong Hu, National Taiwan Univ. Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

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