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Journal of Biomedical Optics

Lookup-table method for imaging optical properties with structured illumination beyond the diffusion theory regime
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Paper Abstract

Sinusoidally structured illumination is used in concert with a phantom-based lookup-table (LUT) to map wide-field optical properties in turbid media with reduced albedos as low as 0.44. A key advantage of the lookup-table approach is the ability to measure the absorption (µa) and reduced scattering coefficients (µ′s) over a much broader range of values than permitted by current diffusion theory methods. Through calibration with a single reflectance standard, the LUT can extract µ′s from 0.8 to 2.4 mm-1 with an average root-mean-square (rms) error of 7% and extract µa from 0 to 1.0 mm-1 with an average rms error of 6%. The LUT is based solely on measurements of two parameters, reflectance R and modulation M at an illumination period of 10 mm. A single set of three phase-shifted images is sufficient to measure both M and R, which are then used to generate maps of absorption and scattering by referencing the LUT. We establish empirically that each pair (M,R) maps uniquely to only one pair of (µ′sa) and report that the phase function (i.e., size) of the scatterers can influence the accuracy of optical property extraction.

Paper Details

Date Published: 1 May 2010
PDF: 9 pages
J. Biomed. Opt. 15(3) 036013 doi: 10.1117/1.3431728
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Tim A. Erickson, The Univ. of Texas at Austin (United States)
Amaan Mazhar, Beckman Laser Institute and Medical Clinic (United States)
David J. Cuccia, Beckman Laser Institute and Medical Clinic (United States)
Anthony J. Durkin, Beckman Laser Institute and Medical Clinic (United States)
James W. Tunnell, The Univ. of Texas at Austin (United States)

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