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Journal of Biomedical Optics • Open Access

Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy
Author(s): Björn Kemper; Andreas Bauwens; Angelika Vollmer; Steffi Ketelhut; Patrik Langehanenberg; Johannes Muthing; Helge Karch; Gert von Bally

Paper Abstract

Digital holographic microscopy (DHM) enables quantitative multifocus phase contrast imaging for nondestructive technical inspection and live cell analysis. Time-lapse investigations on human brain microvascular endothelial cells demonstrate the use of DHM for label-free dynamic quantitative monitoring of cell division of mother cells into daughter cells. Cytokinetic DHM analysis provides future applications in toxicology and cancer research.

Paper Details

Date Published: 1 May 2010
PDF: 6 pages
J. Biomed. Opt. 15(3) 036009 doi: 10.1117/1.3431712
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Björn Kemper, Westfälische Wilhelms-Univ. Münster (Germany)
Andreas Bauwens, Westfälische Wilhelms-Univ Münster (Germany)
Angelika Vollmer, Westfälische Wilhelms-Univ Münster (Germany)
Steffi Ketelhut, Westfälische Wilhelms-Univ. Münster (Germany)
Patrik Langehanenberg, Westfälische Wilhelms-Univ Münster (Germany)
Johannes Muthing, Westfälische Wilhelms-Univ. Münster (Germany)
Helge Karch, Westfälische Wilhelms-Univ. Münster (Germany)
Gert von Bally, Westfälische Wilhelms-Univ. Münster (Germany)

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