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Journal of Biomedical Optics • Open Access

Characterization of tumor microvascular structure and permeability: comparison between magnetic resonance imaging and intravital confocal imaging
Author(s): Nina K. Reitan; Marte Thuen; Pål Erik Goa; Catharina de Lange Davies

Paper Abstract

Solid tumors are characterized by abnormal blood vessel organization, structure, and function. These abnormalities give rise to enhanced vascular permeability and may predict therapeutic responses. The permeability and architecture of the microvasculature in human osteosarcoma tumors growing in dorsal window chambers in athymic mice were measured by confocal laser scanning microscopy (CLSM) and dynamic contrast enhanced magnetic resonance imaging (DCE-MRI). Dextran (40 kDa) and Gadomer were used as molecular tracers for CLSM and DCE-MRI, respectively. A significant correlation was found between permeability indicators. The extravasation rate Ki as measured by CLSM correlated positively with DCE-MRI parameters, such as the volume transfer constant Ktrans and the initial slope of the contrast agent concentration-time curve. This demonstrates that these two techniques give complementary information. Extravasation was further related to microvascular structure and was found to correlate with the fractal dimension and vascular density. The structural parameter values that were obtained from CLSM images were higher for abnormal tumor vasculature than for normal vessels.

Paper Details

Date Published: 1 May 2010
PDF: 11 pages
J. Biomed. Opt. 15(3) 036004 doi: 10.1117/1.3431095
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Nina K. Reitan, Norwegian Univ. of Science and Technology (Norway)
Marte Thuen, Norwegian Univ. of Science and Technology (Norway)
Pål Erik Goa, St. Olavs Hospital (Norway)
Catharina de Lange Davies, Norwegian Univ. of Science and Technology (Norway)

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