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Journal of Biomedical Optics

Transient transfection of mammalian cells using a violet diode laser
Author(s): Maria Leilani Y. Torre-Mapa; Liselotte Veldhuis; Martin Ploschner; Kishan Dholakia; Frank James Gunn-Moore
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Paper Abstract

We demonstrate the first use of the violet diode laser for transient mammalian cell transfection. In contrast to previous studies, which showed the generation of stable cell lines over a few weeks, we develop a methodology to transiently transfect cells with an efficiency of up to ~40%. Chinese hamster ovary (CHO-K1) and human embryonic kidney (HEK293) cells are exposed to a tightly focused 405-nm laser in the presence of plasmid DNA encoding for a mitochondrial targeted red fluorescent protein. We report transfection efficiencies as a function of laser power and exposure time for our system. We also show, for the first time, that a continuous wave laser source can be successfully applied to selective gene silencing experiments using small interfering RNA. This work is a major step towards an inexpensive and portable phototransfection system.

Paper Details

Date Published: 1 July 2010
PDF: 7 pages
J. Biomed. Opt. 15(4) 041506 doi: 10.1117/1.3430730
Published in: Journal of Biomedical Optics Volume 15, Issue 4
Show Author Affiliations
Maria Leilani Y. Torre-Mapa, Univ. of St. Andrews (United Kingdom)
Liselotte Veldhuis, Univ. of St. Andrews (United Kingdom)
Martin Ploschner, Univ. of St. Andrews (United Kingdom)
Kishan Dholakia, Univ. of St. Andrews (United Kingdom)
Frank James Gunn-Moore, Univ. of St. Andrews (United Kingdom)

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