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Journal of Biomedical Optics • Open Access

Virtual four-dimensional imaging of lung parenchyma by optical coherence tomography in mice
Author(s): Sven Meissner; Arata Tabuchi; Michael Mertens; Wolfgang Michael Kuebler; Edmund Koch

Paper Abstract

In this feasibility study, we present a method for virtual 4-D imaging of healthy and injured subpleural lung tissue in the ventilated mouse. We use triggered swept source optical coherence tomography (OCT) with an A-scan frequency of 20 kHz to image murine subpleural alveoli during the inspiratory phase. The data acquisition is gated to the ventilation pressure to take single B-scans in each respiration cycle for different pressure levels. The acquired B-scans are combined off-line into one volume scan for each pressure level. The air fraction in healthy lungs and injured lungs is measured using 2-D OCT en-face images. Upon lung inspiration from 2 to 12 cmH2O ventilation pressure, the air fraction increases in healthy lungs by up to 11% and in injured lungs by 8%. This expansion correlates well with results of previous studies, reporting increased alveolar area with increased ventilation pressures. We demonstrate that OCT is a useful tool to investigate alveolar dynamics in spatial dimensions.

Paper Details

Date Published: 1 May 2010
PDF: 4 pages
J. Biomed. Opt. 15(3) 036016 doi: 10.1117/1.3425654
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Sven Meissner, Univ. Hospital Carl Gustav Carus Dresden (Germany)
Arata Tabuchi
Michael Mertens, Charité Universitätsmedizin Berlin (Germany)
Wolfgang Michael Kuebler, Charité Universitätsmedizin Berlin (Germany)
Edmund Koch, Technische Univ. Dresden (Germany)

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