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Journal of Biomedical Optics • Open Access

Multiphoton microscopy with near infrared contrast agents

Paper Abstract

While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

Paper Details

Date Published: 1 May 2010
PDF: 3 pages
J. Biomed. Opt. 15(3) 030505 doi: 10.1117/1.3420209
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Siavash Yazdanfar, GE Global Research (United States)
Chulmin Joo, GE Global Research (United States)
Chun Zhan, GE Global Research (United States)
Mikhail Berezin, Washington Univ. in St. Louis (United States)
Walter J. Akers, Washington Univ. in St. Louis (United States)
Samuel Achilefu, Washington Univ. in St. Louis (United States)


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