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Journal of Biomedical Optics • Open Access

Reflective interferometric chamber for quantitative phase imaging of biological sample dynamics
Author(s): Natan Tzvi Shaked; Yizheng Zhu; Nima Badie; Nenad Bursac; Adam P. Wax

Paper Abstract

We introduce a new interferometric setup for single-exposure wide-field holographic phase imaging of highly dynamic biological samples. In this setup, the interferometric signal originates from a specially designed reflective interferometric chamber (InCh), creating an off-axis interferogram on the output plane of the system. The setup only requires the InCh and a simple reflection-mode two lens imaging system, without the need for additional optical elements such as gratings in the beam path. In addition, due to the close-to-common-path geometry of the setup, phase noise is greatly reduced. We experimentally compare the inherent phase stability of the system in ambient conditions to that of a conventional interferometer. We also demonstrate use of this system for wide-field quantitative phase imaging of two different highly dynamic, optically transparent biological samples: beating myocardial cells and moving unicellular microorganisms.

Paper Details

Date Published: 1 May 2010
PDF: 3 pages
J. Biomed. Opt. 15(3) 030503 doi: 10.1117/1.3420179
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Natan Tzvi Shaked, Duke Univ. (United States)
Yizheng Zhu, Duke Univ. (United States)
Nima Badie, Duke Univ. (United States)
Nenad Bursac, Duke Univ. (United States)
Adam P. Wax, Duke Univ. (United States)

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