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Journal of Biomedical Optics • Open Access

In vivo early diagnosis of gastric dysplasia using narrow-band image-guided Raman endoscopy
Author(s): Zhiwei Huang; Mads Sylvest Bergholt; Wei Zheng; Kan Lin; Khek-Yu Ho; Ming The; Khay-Guan Yeoh

Paper Abstract

We first report on the implementation of a novel narrow-band image-guided Raman endoscopy technique for in vivo diagnosis of gastric dysplasia. High-quality in vivo Raman spectra can be acquired from normal and dysplastic gastric mucosal tissue within 0.5 sec under narrow-band image (NBI) guidance at gastroscopy. Significant differences are observed in in vivo Raman spectra between normal (n=54) and dysplastic (n=18) gastric tissue from 30 gastric patients, particularly in the spectral ranges of 825 to 950, 1000 to 1100, 1250 to 1500, and 1600 to 1800 cm−1, which primarily contain signals related to proteins, nucleic acids, and lipids. The multivariate analysis [i.e., principal components analysis (PCA) and linear discriminant analysis (LDA)], together with the leave-one tissue site-out, cross validation on in vivo gastric Raman spectra yields a diagnostic sensitivity of 94.4% (17/18) and specificity of 96.3% (52/54) for distinction of gastric dysplastic tissue. This study suggests that narrowband image-guided Raman endoscopy associated with PCA-LDA diagnostic algorithms has potential for the noninvasive, in vivo early diagnosis and detection of gastric precancer during clinical gastroscopic examination.

Paper Details

Date Published: 1 May 2010
PDF: 5 pages
J. Biomed. Opt. 15(3) 037017 doi: 10.1117/1.3420115
Published in: Journal of Biomedical Optics Volume 15, Issue 3
Show Author Affiliations
Zhiwei Huang, National Univ. of Singapore (Singapore)
Mads Sylvest Bergholt, National Univ. of Singapore (Singapore)
Wei Zheng, National Univ. of Singapore (Singapore)
Kan Lin, National Univ. of Singapore (Singapore)
Khek-Yu Ho, National Univ. of Singapore (Singapore)
Ming The, National Univ. of Singapore (Singapore)
Khay-Guan Yeoh, National Univ. of Singapore (Singapore)

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