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Journal of Micro/Nanolithography, MEMS, and MOEMS

Effects of a glutaraldehyde cross-linking layer on a quartz crystal microbalance−based α-fetoprotein biosensor with cystamine self-assembly monolayer
Author(s): I-Yu Huang; Chang-Yu Lin; En-Chang Wu
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Paper Abstract

To identify the extreme low concentration of alpha-fetoprotein (AFP) antigen in human serum for early detection of hepatocellular carcinoma, we aim to develop a high-sensitivity and low-cost AFP biosensor using quartz crystal microbalance (QCM) and cystamine self-assembly monolayer (SAM) technologies. In this study, the surface topographies of concentrations (0.1 and 1.0 mg/mL) of AFP antibody with and without 1.25% glutaraldehyde cross-linking layer will be analyzed by an atomic force microscope system to investigate the effects of the glutaraldehyde layer on the sensing characteristics of the QCM-based AFP biosensor. According to our experimental results, the mass sensitivity was improved almost doubly (from 0.07 to 0.146 Hz mL mg−1) as the glutaraldehyde layer was added between the 20-mM cystamine SAM and the low-concentration (0.1 mg/mL) AFP antibody. Either with or without the glutaraldehyde layer, higher mass sensitivity (0.163 to 0.335 Hz mL mg−1) was obtained as the AFP antibody concentration increased to 1.0 mg/mL. However, a large AFP antibody requirement will increase fabrication cost and limit disposable application. We also demonstrated that high sensing linearities (95.67 to 99.6%) of the QCM-AFP biosensors can be achieved without being obviously affected by the glutaraldehyde layer and AFP antibody concentration.

Paper Details

Date Published: 1 April 2010
PDF: 7 pages
J. Micro/Nanolith. 9(2) 023008 doi: 10.1117/1.3415495
Published in: Journal of Micro/Nanolithography, MEMS, and MOEMS Volume 9, Issue 2
Show Author Affiliations
I-Yu Huang, National Sun Yat-Sen Univ. (Taiwan)
Chang-Yu Lin, Industrial Technology Research Institute (Taiwan)
En-Chang Wu, National Sun Yat-Sen Univ. (Taiwan)

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