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Journal of Biomedical Optics

Using surface-enhanced Raman spectroscopy to probe for genetic markers on single-stranded DNA
Author(s): Benjamin Moody; John Leotaud; Gregory S. McCarty
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Paper Abstract

Methods capable of quickly and inexpensively collecting genetic information are of increasing importance. We report a method of using surface-enhanced Raman spectroscopy to probe single-stranded DNA for genetic markers. This unique approach is used to analyze unmodified genes of moderate length for genetic markers by hybridizing native test oligonucleotides into a surface-enhanced Raman complex, vastly increasing detection sensitivity as compared to traditional Raman spectroscopy. The Raman complex is formed by sandwiching the test DNA between 40-nm gold nanoparticles and a photolithographically defined gold surface. With this design, we are able to collect characteristic Raman spectra about the test DNA and to detect genetic markers such as single-nucleotide polymorphisms (SNPs) and polymorphic regions. Results show that strands containing one of three different types of polymorphism can be differentiated using statistically significant trends regarding Raman intensity.

Paper Details

Date Published: 1 March 2010
PDF: 6 pages
J. Biomed. Opt. 15(2) 027014 doi: 10.1117/1.3400702
Published in: Journal of Biomedical Optics Volume 15, Issue 2
Show Author Affiliations
Benjamin Moody, North Carolina State Univ. (United States)
John Leotaud, The Univ. of North Carolina at Chapel Hill (United States)
Gregory S. McCarty, North Carolina State Univ. (United States)

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