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Journal of Biomedical Optics

Nonlinear multicontrast microscopy of hematoxylin-and-eosin-stained histological sections
Author(s): Adam E. Tuer; Danielle Tokarz; Nicole Prent; Richard Cisek; Jennifer Alami; Daniel J. Dumont; Ludmilla Bakueva; John A. Rowlands; Virginijus Barzda
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Paper Abstract

Imaging hematoxylin-and-eosin-stained cancerous histological sections with multicontrast nonlinear excitation fluorescence, second- and third-harmonic generation (THG) microscopy reveals cellular structures with extremely high image contrast. Absorption and fluorescence spectroscopy together with second hyperpolarizability measurements of the dyes shows that strong THG appears due to neutral hemalum aggregation and is subsequently enhanced by interaction with eosin. Additionally, fluorescence lifetime imaging microscopy reveals eosin fluorescence quenching by hemalums, showing better suitability of only eosin staining for fluorescence microscopy. Multicontrast nonlinear microscopy has the potential to differentiate between cancerous and healthy tissue at a single cell level.

Paper Details

Date Published: 1 March 2010
PDF: 9 pages
J. Biomed. Opt. 15(2) 026018 doi: 10.1117/1.3382908
Published in: Journal of Biomedical Optics Volume 15, Issue 2
Show Author Affiliations
Adam E. Tuer, Univ. of Toronto Mississauga (Canada)
Danielle Tokarz, Univ. of Toronto Mississauga (Canada)
Nicole Prent, Univ. of Toronto Mississauga (Canada)
Richard Cisek, Univ. of Toronto Mississauga (Canada)
Jennifer Alami, Sunnybrook and Women's Health Sciences Ctr. (Canada)
Daniel J. Dumont, Sunnybrook and Women's Health Sciences Ctr. (Canada)
Ludmilla Bakueva, Sunnybrook and Women's Health Sciences Ctr. (Canada)
John A. Rowlands, Thunder Bay Regional Health Sciences Ctr. (Canada)
Virginijus Barzda, Univ. of Toronto Mississauga (Canada)

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