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Journal of Biomedical Optics

Digital holographic microscopy real-time monitoring of cytoarchitectural alterations during simulated microgravity
Author(s): Christophe Pache; Jonas Kühn; Kriss Westphal; Muhammed Fatih Toy; Jérôme Parent; Oralea Buchi; Alfredo Franco-Obregon; Christian D. Depeursinge; Marcel Egli
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Paper Abstract

Previous investigations on mammalian cells have shown that microgravity, either that experienced in space, or simulated on earth, causes severe cellular modifications that compromise tissue determination and function. The aim of this study is to investigate, in real time, the morphological changes undergone by cells experiencing simulated microgravity by using digital holographic microscopy (DHM). DHM analysis of living mouse myoblasts (C2C12) is undertaken under simulated microgravity with a random positioning machine. The DHM analysis reveals cytoskeletal alterations similar to those previously reported with conventional methods, and in agreement with conventional brightfield fluorescence microscopy a posteriori investigation. Indeed, DHM is shown to be able to noninvasively and quantitatively detect changes in actin reticular formation, as well as actin distribution, in living unstained samples. Such results were previously only obtainable with the use of labeled probes in conjunction with conventional fluorescence microscopy, with all the classically described limitations in terms of bias, bleaching, and temporal resolution.

Paper Details

Date Published: 1 March 2010
PDF: 9 pages
J. Biomed. Opt. 15(2) 026021 doi: 10.1117/1.3377960
Published in: Journal of Biomedical Optics Volume 15, Issue 2
Show Author Affiliations
Christophe Pache, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Jonas Kühn, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Kriss Westphal, ETH Zurich (Switzerland)
Muhammed Fatih Toy
Jérôme Parent, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Oralea Buchi, ETH Zurich (Switzerland)
Alfredo Franco-Obregon, ETH Zurich (Switzerland)
Christian D. Depeursinge, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Marcel Egli, ETH Zurich (Switzerland)

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