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Journal of Biomedical Optics • Open Access

Rapid near-infrared fluorescence excitation-emission matrix spectroscopy for multifluorophore characterization using an acousto-optic tunable filter technique
Author(s): Hao Li; Wei Zheng; Zhiwei Huang

Paper Abstract

We report on a novel acousto-optic tunable filter (AOTF)-based near-infrared (NIR) fluorescence excitation-emission matrix (EEM) spectroscopy technique for rapid multifluorophore characterization. We implement a unique light filtering module design by using cascaded AOTFs coupled with three orthogonally oriented polarizers to effectively remove the side-ripple artifacts of AOTFs as well as by using a pair of AOTFs coupled with two orthogonally oriented polarizers to improve detection efficiency for high-quality fluorescence EEM acquisitions. NIR fluorescence EEM spectroscopy (41 excitation wavelengths ranging from 550 to 950 nm in 10-nm increments; fluorescence emission from 570 to 1000 nm at 10-nm intervals) can be acquired from fluorescence dyes [e.g., diethylthiatricarbocyanine (DTTC) iodide, oxazine 750, and IR 140] within 10 s or even less, illustrating the potential of the AOTF-based NIR EEM technique developed for rapid multifluorophore analysis and characterization in biochemical and biomedical systems.

Paper Details

Date Published: 1 March 2010
PDF: 4 pages
J. Biomed. Opt. 15(2) 027010 doi: 10.1117/1.3374051
Published in: Journal of Biomedical Optics Volume 15, Issue 2
Show Author Affiliations
Hao Li, National Univ. of Singapore (Singapore)
Wei Zheng, National Univ. of Singapore (Singapore)
Zhiwei Huang, National Univ. of Singapore (Singapore)

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