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Journal of Biomedical Optics • Open Access

Intramyocardial oxygen transport by quantitative diffuse reflectance spectroscopy in calves
Author(s): Tobias Lindbergh; Marcus Larsson; Zoltán Szabó; Henrik Casimir-Ahn; Tomas Strömberg

Paper Abstract

Intramyocardial oxygen transport was assessed during open-chest surgery in calves by diffuse reflectance spectroscopy using a small intramuscular fiber-optic probe. The sum of hemo- and myoglobin tissue fraction and oxygen saturation, the tissue fraction and oxidation of cytochrome aa3, and the tissue fraction of methemoglobin were estimated using a calibrated empirical light transport model. Increasing the oxygen content in the inhaled gas, 21%-50%-100%, in five calves (group A) gave an increasing oxygen saturation of 19±4%, 24±5%, and 28±8% (p<0.001, ANOVA repeated measures design) and mean tissue fractions of 1.6% (cytochrome aa3) and 1.1% (hemo- and myoglobin). Cardiac arrest in two calves gave an oxygen saturation lower than 5%. In two calves (group B), a left ventricular assistive device (LVAD pump) was implanted. Oxygen saturation in group B animals increased with LVAD pump speed (p<0.001, ANOVA) and with oxygen content in inhaled gas (p<0.001, ANOVA). The cytochrome aa3 oxidation level was above 96% in both group A and group B calves, including the two cases involving cardiac arrest. In conclusion, the estimated tissue fractions and oxygenation/oxidation levels of the myocardial chromophores during respiratory and hemodynamic provocations were in agreement with previously presented results, demonstrating the potential of the method.

Paper Details

Date Published: 1 March 2010
PDF: 11 pages
J. Biomed. Opt. 15(2) 027009 doi: 10.1117/1.3374050
Published in: Journal of Biomedical Optics Volume 15, Issue 2
Show Author Affiliations
Tobias Lindbergh, Linköping Univ. (Sweden)
Marcus Larsson, Linköping Univ. (Sweden)
Zoltán Szabó, Linköping Univ. (Sweden)
Henrik Casimir-Ahn, Univ. Hospital Linköping (Sweden)
Tomas Strömberg, Linköping Univ. (Sweden)

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