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Journal of Biomedical Optics • Open Access

System development for high frequency ultrasound-guided fluorescence quantification of skin layers
Author(s): Josiah D. Gruber; Akshat Paliwal; Venkat Krishnaswamy; Hamid R. Ghadyani; Michael Jermyn; Julie A. O'Hara; Scott C. Davis; Joanna S. Kerley-Hamilton; Nicholas Shworak; Edward V. Maytin; Tayyaba Hasan; Brian W. Pogue

Paper Abstract

A high frequency ultrasound-coupled fluorescence tomography system, primarily designed for imaging of protoporphyrin IX production in skin tumors in vivo, is demonstrated for the first time. The design couples fiber-based spectral sampling of the protoporphyrin IX fluorescence emission with high frequency ultrasound imaging, allowing thin-layer fluorescence intensities to be quantified. The system measurements are obtained by serial illumination of four linear source locations, with parallel detection at each of five interspersed detection locations, providing 20 overlapping measures of subsurface fluorescence from both superficial and deep locations in the ultrasound field. Tissue layers are defined from the segmented ultrasound images and diffusion theory used to estimate the fluorescence in these layers. The system calibration is presented with simulation and phantom validation of the system in multilayer regions. Pilot in-vivo data are also presented, showing recovery of subcutaneous tumor tissue values of protoporphyrin IX in a subcutaneous U251 tumor, which has less fluorescence than the skin.

Paper Details

Date Published: 1 March 2010
PDF: 5 pages
J. Biomed. Opt. 15(2) 026028 doi: 10.1117/1.3374040
Published in: Journal of Biomedical Optics Volume 15, Issue 2
Show Author Affiliations
Josiah D. Gruber, Dartmouth College (United States)
Akshat Paliwal, Case Western Reserve Univ. (United States)
Venkat Krishnaswamy, Dartmouth College (United States)
Hamid R. Ghadyani, Dartmouth College (United States)
Michael Jermyn
Julie A. O'Hara
Scott C. Davis, Dartmouth College (United States)
Joanna S. Kerley-Hamilton
Nicholas Shworak
Edward V. Maytin, Cleveland Clinic Lerner Research Institute (United States)
Tayyaba Hasan, Wellman Ctr. for Photomedicine (United States)
Brian W. Pogue, Dartmouth College (United States)

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