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Journal of Biomedical Optics

Diffusion and cellular uptake of drugs in live cells studied with surface-enhanced Raman scattering probes
Author(s): Stefan Bálint; Satish Rao; Mónica Marro Sánchez; Veronika Huntošová; Pavol Miškovský; Dmitri Petrov
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Paper Abstract

An understanding of the mechanisms of drug diffusion and uptake through cellular membranes is critical for elucidating drug action and in the development of effective drug delivery systems. We study these processes for emodin, a potential anticancer drug, in live cancer cells using surface-enhanced Raman scattering. Micrometer-sized silica beads covered by nanosized silver colloids are passively embedded into the cell and used as sensors of the drug. We demonstrate that the technique offers distinct advantages: the possibility to study the kinetics of drug diffusion through the cellular membrane toward specific cell organelles, the detection of lower drug concentrations compared to fluorescence techniques, and less damage imparted on the cell.

Paper Details

Date Published: 1 March 2010
PDF: 7 pages
J. Biomed. Opt. 15(2) 027005 doi: 10.1117/1.3369844
Published in: Journal of Biomedical Optics Volume 15, Issue 2
Show Author Affiliations
Stefan Bálint, ICFO - Instituto de Ciencias Fotónicas (Spain)
Satish Rao, ICFO - Instituto de Ciencias Fotónicas (Spain)
Mónica Marro Sánchez, ICFO - Instituto de Ciencias Fotónicas (Spain)
Veronika Huntošová, Pavol Jozef Šafárik Univ. in Kolšice (Slovakia)
Pavol Miškovský, Pavol Jozef Šafárik Univ. in Kolšice (Slovakia)
Dmitri Petrov, ICFO - Instituto de Ciencias Fotónicas (Spain)


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