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Journal of Biomedical Optics • Open Access

Imaging dermal blood flow through the intact rat skin with an optical clearing method
Author(s): Dan Zhu; Jing Wang; Zhongwei Zhi; Xiang Wen; Qingming Luo

Paper Abstract

Noninvasive detection of skin microcirculation is very significant for clinical diagnosis and therapy of peripheral vascular disease. In this study, an optical clearing method enables a laser speckle contrast imaging (LSCI) technique to image dermal blood flow through intact rat skin. The optical clearing effect of rat skin in vitro caused by a mixture of PEG-400 and Thiazone is evaluated by a commercially available spectrophotometer with an integrating sphere. A LSCI system is applied to image the dermal blood flow of in vivo rat skin after topical treatment of the agents. The results show that the reduced scattering coefficient (630 nm) is down to 60% of the initial value after 40 min of topical treatment of the mixture on skin in vitro. In vivo experimental results show that a 12-min treatment of the mixture on the epidermis can make the skin transparent and dermal vessels can be observed, while treatment with pure PEG-400 cannot. The process of skin optical clearing lasts, and the skin can be recovered by treatment of saline solution. Because optical clearing decreases the temporal contrast, dermal blood flow can be obtained by the laser speckle contrast imaging technique.

Paper Details

Date Published: 1 March 2010
PDF: 7 pages
J. Biomed. Opt. 15(2) 026008 doi: 10.1117/1.3369739
Published in: Journal of Biomedical Optics Volume 15, Issue 2
Show Author Affiliations
Dan Zhu, Britton Chance Ctr. for Biomedical Photonics (China)
Jing Wang, Britton Chance Ctr. for Biomedical Photonics (China)
Zhongwei Zhi, Britton Chance Ctr. for Biomedical Photonics (China)
Xiang Wen, Britton Chance Ctr. for Biomedical Photonics (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)

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