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Journal of Biomedical Optics • Open Access

Quantitative measurement of reactive oxygen species in vivo by utilizing a novel method: chemiluminescence with an internal fluorescence as reference

Paper Abstract

Reactive oxygen species (ROS) produced by photodynamic therapy (PDT) is recorded in vivo using a chemiluminescence (CL)-based gated optical system. A novel approach is developed to utilize the fluorescence (FL) of the CL probe as an internal fluorescence to calibrate the observed CL on pharmacokinetics of the probe in situ. The results show that during an in vivo PDT session, the intensity of CL decreases significantly and the decaying of CL is governed by fast and slow time components. By comparing the temporal profile of FL to that of the corresponding CL, it is found that the slow component is mainly attributed to the probe pharmacokinetics, whereas the fast component is likely due to rapid oxygen consumption as a result of PDT treatment. With carefully selected criteria, it is possible to minimize the effect of probe pharmacokinetics. This significantly improves the monitoring method for practical applications.

Paper Details

Date Published: 1 March 2010
PDF: 7 pages
J. Biomed. Opt. 15(2) 027006 doi: 10.1117/1.3368688
Published in: Journal of Biomedical Optics Volume 15, Issue 2
Show Author Affiliations
Yanchun Wei, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Shiming Luo, South China Normal Univ. (China)
Liyong Yang, South China Normal Univ. (China)
Qun Chen, South China Normal Univ. (China)


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