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Journal of Biomedical Optics

Monitoring human leukocyte antigen class I molecules by micro-Raman spectroscopy at single-cell level
Author(s): Gobind Das; Rosanna La Rocca; Tadepally Lakshmikanth; Francesco Gentile; Rossana Tallerico; Lia Zambetti; James Devitt; Patrizio Candeloro; Francesco De Angelis; Ennio Carbone; Enzo M. Di Fabrizio
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Paper Abstract

Human leukocyte antigen (HLA) class I molecules are formed by three immunoglobulin-like domains (α1, α2, and α3) once folded by peptide and β2-microglobulin show the presence of two α-helix streams and one β-sheet limiting the pocket for the antigenic peptide. The loss of HLA class I expression in tumors and virus-infected cells, on one hand, prevents T cell recognition, while on the other hand, it leads to natural killer (NK) cell mediated cytotoxicity. We propose the possibility of using Raman spectroscopy to measure the relative expression of HLA class I molecules at the single-cell level. Raman spectra are recorded for three cell lines (K562, T2, and T3) and monomers (HLA class I folded, unfolded and peptide+β2-microlobulin refolded) using 830 nm laser line. Our data are consistent with the hypothesis that in the Raman spectra, ranging from 1600 to 1800 cm-1, the intensity variation of cells associated with HLA class I molecules could be measured.

Paper Details

Date Published: 1 March 2010
PDF: 9 pages
J. Biomed. Opt. 15(2) 027007 doi: 10.1117/1.3368687
Published in: Journal of Biomedical Optics Volume 15, Issue 2
Show Author Affiliations
Gobind Das, Univ. degli studi Magna Græcia di Catanzaro (Italy)
Rosanna La Rocca, Univ. degli studi Magna Græcia di Catanzaro (Italy)
Tadepally Lakshmikanth, Karolinska Institutet (Sweden)
Francesco Gentile, Univ. degli studi Magna Græcia di Catanzaro (Italy)
Rossana Tallerico, Univ. degli studi Magna Græcia di Catanzaro (Italy)
Lia Zambetti, The Royal Free Hospital (United Kingdom)
James Devitt, The Royal Free Hospital (United Kingdom)
Patrizio Candeloro, Univ. degli studi Magna Græcia di Catanzaro (Italy)
Francesco De Angelis, Univ. degli studi Magna Græcia di Catanzaro (Italy)
Ennio Carbone, Karolinska Institutet (Sweden)
Enzo M. Di Fabrizio, Univ. degli studi Magna Græcia di Catanzaro (Italy)


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