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Journal of Biomedical Optics • Open Access

Arterial radius estimation from microscopic data using a new algorithm for circle parameter estimation
Author(s): Han J.W. van Triest; Remco T. A. Megens; Hans C. van Assen; Bart M. ter Haar Romeny; Marc A. M. J. Van Zandvoort

Paper Abstract

We develop and test a new method for automatic determination of vessel wall diameters from image stacks obtained using two-photon laser scanning microscopy (TPLSM) on viable arteries in perfusion flow chambers. To this extent, a new method is proposed for estimating the parameters of a circle describing the inner diameter of the blood vessels. The new method is based on the Hough transform and the observation that three points that are not colinear uniquely define a circle. By only storing the estimated center location, the computational and memory costs of the Hough transform can be greatly reduced. We test the algorithm on 20 images and compare the result with a ground-truth established by human volunteers and a standard least-squares technique. With errors of 3 to 5%, the algorithm enables accurate estimation of the vessel diameters from image stacks containing only small parts of the vessel cross section. Combined with TPLSM imaging of anatomical vessel wall properties, potentially, the algorithm enables the correlation of structural and functional properties of large intact arteries simultaneously, without requirements for additional experiments.

Paper Details

Date Published: 1 March 2010
PDF: 8 pages
J. Biomed. Opt. 15(2) 026012 doi: 10.1117/1.3368679
Published in: Journal of Biomedical Optics Volume 15, Issue 2
Show Author Affiliations
Han J.W. van Triest, Northeastern Univ. (China)
Remco T. A. Megens, RWTH Aachen (Germany)
Hans C. van Assen, Technische Univ. Eindhoven (Netherlands)
Bart M. ter Haar Romeny, Technische Univ. Eindhoven (Netherlands)
Marc A. M. J. Van Zandvoort, Univ. Maastricht (Netherlands)


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