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Journal of Biomedical Optics • Open Access

Multimodal nonlinear optical imaging of atherosclerotic plaque development in myocardial infarction-prone rabbits
Author(s): Alex C. T. Ko; Andrew Ridsdale; Michael S. D. Smith; Leila B. Mostaço-Guidolin; Mark D. Hewko; Adrian F. Pegoraro; Elicia M. Kohlenberg; Bernhard J. Schattka; Masashi Shiomi; Albert Stolow; Michael G. Sowa

Paper Abstract

Label-free imaging of bulk arterial tissue is demonstrated using a multimodal nonlinear optical microscope based on a photonic crystal fiber and a single femtosecond oscillator operating at 800 nm. Colocalized imaging of extracellular elastin fibers, fibrillar collagen, and lipid-rich structures within aortic tissue obtained from atherosclerosis-prone myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits is demonstrated through two-photon excited fluorescence, second harmonic generation, and coherent anti-Stokes Raman scattering, respectively. These images are shown to differentiate healthy arterial wall, early atherosclerotic lesions, and advanced plaques. Clear pathological changes are observed in the extracellular matrix of the arterial wall and correlated with progression of atherosclerotic disease as represented by the age of the WHHLMI rabbits.

Paper Details

Date Published: 1 March 2010
PDF: 3 pages
J. Biomed. Opt. 15(2) 020501 doi: 10.1117/1.3353960
Published in: Journal of Biomedical Optics Volume 15, Issue 2
Show Author Affiliations
Alex C. T. Ko, National Research Council Canada (Canada)
Andrew Ridsdale, National Research Council Canada (Canada)
Michael S. D. Smith, National Research Council Canada (Canada)
Leila B. Mostaço-Guidolin
Mark D. Hewko, National Research Council Canada (Canada)
Adrian F. Pegoraro, National Research Council Canada (Canada)
Elicia M. Kohlenberg, National Research Council Canada (Canada)
Bernhard J. Schattka, National Research Council Canada (Canada)
Masashi Shiomi, Kobe Univ. School of Medicine (Japan)
Albert Stolow, National Research Council Canada (Canada)
Michael G. Sowa, National Research Council Canada (Canada)


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