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Journal of Biomedical Optics

Photoacoustic microtomography using optical interferometric detection
Author(s): Robert Nuster; Markus Holotta; Christian Kremser; Harald Grossauer; Peter Burgholzer; Günther Paltauf
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Paper Abstract

A device for three-dimensional (3-D) photoacoustic tomography with resolution in the range of tens of micrometers is presented that uses a light beam for interferometric detection of acoustic waves. Reconstruction of the 3-D initial pressure distribution from the signals representing line integrals of the acoustic field is a two-step process. It uses an inversion of 2-D wave propagation to obtain line projections of the initial pressure distribution and the inverse Radon transform. The light beam, propagating freely in a water bath, is scanned either in an πarc- or box-shaped curve around the object. Simulations are performed to compare the two scanning procedures. The projection images are obtained either using the filtered back projection algorithm for the -arc scanning mode or the frequency domain algorithm for the box scanning mode. While the former algorithm provides slightly better image quality, the latter is about 20 times faster. The ability of the photoacoustic tomography device to create 3-D images with constant resolution throughout the reconstruction volume is demonstrated experimentally using a human hair phantom. These measurements revealed a 3-D resolution below 100 µm. In a second experiment, 3-D imaging of an isolated mouse heart is demonstrated to show the applicability for preclinical and biological research.

Paper Details

Date Published: 1 March 2010
PDF: 6 pages
J. Biomed. Opt. 15(2) 021307 doi: 10.1117/1.3333547
Published in: Journal of Biomedical Optics Volume 15, Issue 2
Show Author Affiliations
Robert Nuster, Karl-Franzens-Univ. Graz (Austria)
Markus Holotta, Innsbruck Medical Univ. (Austria)
Christian Kremser, Univ. Hospital Innsbruck (Austria)
Harald Grossauer, Leopold-Franzens-Univ. Innsbruck (Austria)
Peter Burgholzer, RECENDT GmbH (Austria)
Günther Paltauf, Karl-Franzens-Univ. Graz (Austria)

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