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Journal of Biomedical Optics • Open Access

Cellular resolution ex vivo imaging of gastrointestinal tissues with optical coherence microscopy
Author(s): Aaron D. Aguirre; Yu Chen; Bradley Bryan; Hiroshi Mashimo; Qin Huang; James L. Connolly; James G. Fujimoto

Paper Abstract

Optical coherence microscopy (OCM) combines confocal microscopy and optical coherence tomography (OCT) to improve imaging depth and contrast, enabling cellular imaging in human tissues. We aim to investigate OCM for ex vivo imaging of upper and lower gastrointestinal tract tissues, to establish correlations between OCM imaging and histology, and to provide a baseline for future endoscopic studies. Co-registered OCM and OCT imaging were performed on fresh surgical specimens and endoscopic biopsy specimens, and images were correlated with histology. Imaging was performed at 1.06-µm wavelength with <2-μm transverse and <4-μm axial resolution for OCM, and at 14-μm transverse and <3-μm axial resolution for OCT. Multiple sites on 75 tissue samples from 39 patients were imaged. OCM enabled cellular imaging of specimens from the upper and lower gastrointestinal tracts over a smaller field of view compared to OCT. Squamous cells and their nuclei, goblet cells in Barrett's esophagus, gastric pits and colonic crypts, and fine structures in adenocarcinomas were visualized. OCT provided complementary information through assessment of tissue architectural features over a larger field of view. OCM may provide a complementary imaging modality to standard OCT approaches for endoscopic microscopy.

Paper Details

Date Published: 1 January 2010
PDF: 9 pages
J. Biomed. Opt. 15(1) 016025 doi: 10.1117/1.3322704
Published in: Journal of Biomedical Optics Volume 15, Issue 1
Show Author Affiliations
Aaron D. Aguirre, Massachusetts Institute of Technology (United States)
Yu Chen, Massachusetts Institute of Technology (United States)
Bradley Bryan, Beth Israel Deaconess Medical Ctr. (United States)
Hiroshi Mashimo, VA Boston Healthcare System (United States)
Qin Huang, VA Boston Healthcare System (United States)
James L. Connolly, Beth Israel Deaconess Medical Ctr. (United States)
James G. Fujimoto, Massachusetts Institute of Technology (United States)


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