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Journal of Biomedical Optics

Two-photon intravital imaging of thrombus development
Author(s): Malgorzata Kamocka; Jian Mu; Xiaomin Liu; Nan Chen; Amy Zollman; Barbara Sturonas-Brown; Kenneth Dunn; Zhiliang Xu; Danny Ziyi Chen; Mark S. Alber; Elliot D. Rosen
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Paper Abstract

Thrombus development in mouse mesenteric vessels following laser-induced injury was monitored by high-resolution, near-real-time, two-photon, intravital microscopy. In addition to the use of fluorescently tagged fibrin(ogen) and platelets, plasma was labeled with fluorescently tagged dextran. Because blood cells exclude the dextran in the single plane, blood cells appear as black silhouettes. Thus, in addition to monitoring the accumulation of platelets and fibrin in the thrombus, the protocol detects the movement and incorporation of unlabeled cells in and around it. The developing thrombus perturbs the blood flow near the thrombus surface, which affects the incorporation of platelets and blood cells into the structure. The hemodynamic effects and incorporation of blood cells lead to the development of thrombi with heterogeneous domain structures. Additionally, image processing algorithms and simulations were used to quantify structural features of developing thrombi. This analysis suggests a novel mechanism to stop the growth of developing thrombus.

Paper Details

Date Published: 1 January 2010
PDF: 7 pages
J. Biomed. Opt. 15(1) 016020 doi: 10.1117/1.3322676
Published in: Journal of Biomedical Optics Volume 15, Issue 1
Show Author Affiliations
Malgorzata Kamocka, Indiana Univ. (United States)
Jian Mu, Univ. of Notre Dame (United States)
Xiaomin Liu
Nan Chen, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Amy Zollman, Indiana Univ. (United States)
Barbara Sturonas-Brown, Indiana Univ. (United States)
Kenneth Dunn, Indiana Univ. (United States)
Zhiliang Xu, Univ. of Notre Dame (United States)
Danny Ziyi Chen, Univ. of Notre Dame (United States)
Mark S. Alber, Univ. of Notre Dame (United States)
Elliot D. Rosen, Indiana Univ. (United States)

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