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Journal of Biomedical Optics • Open Access

Spectral imaging reveals microvessel physiology and function from anastomoses to thromboses
Author(s): Mamta Wankhede; Nikita Agarwal; Rodrigo Fraga-Silva; Casey deDeugd; Mohan Raizada; Suh Paul Oh; Brian S. Sorg

Paper Abstract

Abnormal microvascular physiology and function is common in many diseases. Numerous pathologies include hypervascularity, aberrant angiogenesis, or abnormal vascular remodeling among the characteristic features of the disease, and quantitative imaging and measurement of microvessel function can be important to increase understanding of these diseases. Several optical techniques are useful for direct imaging of microvascular function. Spectral imaging is one such technique that can be used to assess microvascular oxygen transport function with high spatial and temporal resolution in microvessel networks through measurements of hemoglobin saturation. We highlight novel observation made with our intravital microscopy spectral imaging system employed with mouse dorsal skin-fold window chambers for imaging hemoglobin saturation in microvessel networks. Specifically, we image acute oxygenation fluctuations in a tumor microvessel network, the development of arteriovenous malformations in a mouse model of hereditary hemorrhagic telangiectasia, and the formation of spontaneous and induced microvascular thromboses and occlusions.

Paper Details

Date Published: 1 January 2010
PDF: 12 pages
J. Biomed. Opt. 15(1) 011111 doi: 10.1117/1.3316299
Published in: Journal of Biomedical Optics Volume 15, Issue 1
Show Author Affiliations
Mamta Wankhede, Univ. of Florida (United States)
Nikita Agarwal, Univ. of Florida (United States)
Rodrigo Fraga-Silva, Univ. of Florida (United States)
Casey deDeugd, Univ. of Florida (United States)
Mohan Raizada, Univ. of Florida (United States)
Suh Paul Oh, Univ. of Florida (United States)
Brian S. Sorg, Univ. of Florida (United States)

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