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Journal of Biomedical Optics • Open Access

Discrimination analysis of human lung cancer cells associated with histological type and malignancy using Raman spectroscopy
Author(s): Yusuke Oshima; Hideyuki Shinzawa; Tatsuji Takenaka; Chie Furihata; Hidetoshi Sato

Paper Abstract

The Raman spectroscopic technique enables the observation of intracellular molecules without fixation or labeling procedures in situ. Raman spectroscopy is a promising technology for diagnosing cancers-especially lung cancer, one of the most common cancers in humans-and other diseases. The purpose of this study was to find an effective marker for the identification of cancer cells and their malignancy using Raman spectroscopy. We demonstrate a classification of cultured human lung cancer cells using Raman spectroscopy, principal component analysis (PCA), and linear discrimination analysis (LDA). Raman spectra of single, normal lung cells, along with four cancer cells with different pathological types, were successfully obtained with an excitation laser at 532 nm. The strong appearance of bands due to cytochrome c (cyt-c) indicates that spectra are resonant and enhanced via the Q-band near 550 nm with excitation light. The PCA loading plot suggests a large contribution of cyt-c in discriminating normal cells from cancer cells. The PCA results reflect the nature of the original cancer, such as its histological type and malignancy. The five cells were successfully discriminated by the LDA.

Paper Details

Date Published: 1 January 2010
PDF: 8 pages
J. Biomed. Opt. 15(1) 017009 doi: 10.1117/1.3316296
Published in: Journal of Biomedical Optics Volume 15, Issue 1
Show Author Affiliations
Yusuke Oshima, Aoyama Gakuin Univ. (Japan)
Hideyuki Shinzawa, RIKEN (Japan)
Tatsuji Takenaka, Aoyama Gakuin Univ. (Japan)
Chie Furihata, Aoyama Gakuin Univ. (Japan)
Hidetoshi Sato, Kwansei Gakuin Univ. (Japan)

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