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Journal of Biomedical Optics

Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope
Author(s): Yiyan Fei; James P. Landry; Yungshin Sun; Xiangdong Zhu; Xiaobing Wang; Juntao Luo; Chun-yi Wu; Kit S. Lam
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Paper Abstract

We describe a high-throughput scanning optical microscope for detecting small-molecule compound microarrays on functionalized glass slides. It is based on measurements of oblique-incidence reflectivity difference and employs a combination of a y-scan galvometer mirror and an x-scan translation stage with an effective field of view of 2 cm×4 cm. Such a field of view can accommodate a printed small-molecule compound microarray with as many as 10,000 to 20,000 targets. The scanning microscope is capable of measuring kinetics as well as endpoints of protein-ligand reactions simultaneously. We present the experimental results on solution-phase protein reactions with small-molecule compound microarrays synthesized from one-bead, one-compound combinatorial chemistry and immobilized on a streptavidin-functionalized glass slide.

Paper Details

Date Published: 1 January 2010
PDF: 8 pages
J. Biomed. Opt. 15(1) 016018 doi: 10.1117/1.3309743
Published in: Journal of Biomedical Optics Volume 15, Issue 1
Show Author Affiliations
Yiyan Fei, Univ. of California, Davis (United States)
James P. Landry, Univ. of California, Davis (United States)
Yungshin Sun, Univ. of California, Davis (United States)
Xiangdong Zhu, Univ. of California, Davis (United States)
Xiaobing Wang, UC Davis Cancer Ctr. (United States)
Juntao Luo, UC Davis Cancer Ctr. (United States)
Chun-yi Wu, UC Davis Cancer Ctr. (United States)
Kit S. Lam, UC Davis Cancer Ctr. (United States)

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