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Journal of Biomedical Optics • Open Access

Efficient mucosal delivery of optical contrast agents using imidazole-modified chitosan
Author(s): Bilal Ghosn; Anne L. van de Ven; Justina O. Tam; Ann Marie Gillenwater; Konstantin V. Sokolov; Rebecca R. Richards-Kortum; Krishnendu Roy

Paper Abstract

The clinical applicability of antibodies and plasmonic nanosensors as topically applied, molecule-specific optical diagnostic agents for noninvasive early detection of cancer and precancer is severely limited by our inability to efficiently deliver macromolecules and nanoparticles through mucosal tissues. We have developed an imidazole-functionalized conjugate of the polysaccharide chitosan (chitosan-IAA) to enhance topical delivery of contrast agents, ranging from small molecules and antibodies to gold nanoparticles up to 44 nm in average diameter. Contrast agent uptake and localization in freshly resected mucosal tissues was monitored using confocal microscopy. Chitosan-IAA was found to reversibly enhance mucosal permeability in a rapid, reproducible manner, facilitating transepithelial delivery of optical contrast agents. Permeation enhancement occurred through an active process, resulting in the delivery of contrast agents via a paracellular or a combined paracellular/transcellular route depending on size. Coadministration of epidermal growth factor receptor-targeted antibodies with chitosan-IAA facilitated specific labeling and discrimination between paired normal and malignant human oral biopsies. Together, these data suggest that chitosan-IAA is a promising topical permeation enhancer for mucosal delivery of optical contrast agents.

Paper Details

Date Published: 1 January 2010
PDF: 11 pages
J. Biomed. Opt. 15(1) 015003 doi: 10.1117/1.3309739
Published in: Journal of Biomedical Optics Volume 15, Issue 1
Show Author Affiliations
Bilal Ghosn, The Univ. of Texas at Austin (United States)
Anne L. van de Ven, Rice Univ. (United States)
Justina O. Tam, The Univ. of Texas at Austin (United States)
Ann Marie Gillenwater, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Konstantin V. Sokolov, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Rebecca R. Richards-Kortum, Rice Univ. (United States)
Krishnendu Roy, The Univ. of Texas at Austin (United States)

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